Molecular Evaluation of the Impact of Nd:YAG Laser and Static Magnetic Field on Genomic DNA of Some Bacterial Isolates using RAPD-PCR

Antimicrobial therapy is frequently associated with the emergence of resistant bacteria a high rate morbidity and mortality worldwide. The present study was aimed at investigating impact neodymium-doped yttrium aluminum (Nd:YAG) laser, static magnetic field (SMF) on cellular growth DNA alteration in some clinical bacterial isolates. Samples from cutaneous wounds were collected by sterile cotton swabs three elderly women admitted to Tikrit Teaching Hospital, City, Iraq. Isolation identification Streptococcus agalactiae, Staphylococcus aureus, Pseudomonas aeruginosa carried out using cultural characteristics, microscopy, biochemical tests. Three broth cultures prepared for each test first culture served as untreated control, second exposed an Nd:YAG laser third SMF. Colony counting done all samples. extracted used perform RAPD-PCR assay. In contrast results showed that radiation more effective than SMF inhibiting Also, caused alteration, which established decreased microbial growth, well development new bands loss original bands. According findings this study, promising technique influencing healing infected wounds. also useful biomarker assay assessing biological bacteria.